epidermal growth factor receptor egfr signaling  (Cell Signaling Technology Inc)

Journal: Cell Reports Medicine

Article Title: Neoadjuvant immunotherapy with or without chemotherapy in locally advanced oral squamous cell carcinoma: Randomized, two-arm, phase 2 trial

doi: 10.1016/j.xcrm.2025.101930

Figure Lengend Snippet: Worse prognosis of non-responders in arm Cam+TPF (A) Spatial heatmaps showing expression intensity of EGFR , including Cam+TPF-pNR group ( n = 2) and Cam-pNR group ( n = 2); scale bar, 5 mm. (B) Dot plot showing expression levels of genes related to EGFR signaling pathway, including Cam+TPF-pNR group ( n = 2) and Cam-pNR group ( n = 2). (C) Heatmap showing the quantitative immunohistochemical results of post-therapy EGFR and downstream proteins in non-responders in arm Cam+TPF compared with arm Cam, including Cam+TPF-pNR group ( n = 2) and Cam-pNR group ( n = 7). (D) Follow-up of the two non-responders in arm Cam+TPF. Red circle indicates primary tumor, red arrow indicates tumor metastasis. EGFR, epidermal growth factor receptor; CT, computed tomography; MRI, magnetic resonance imaging; PET-CT, positron emission tomography and computed tomography.

Article Snippet: Tumor biopsy and surgical samples were collected and examined for a panel of checkpoint molecules, immunocyte subsets and epidermal growth factor receptor (EGFR) signaling proteins using immunohistochemistry, including EGFR (CST, D38B1, 4267S, 1:400), p -EGFR (Tyr1068) (CST, 2234S, 1:200), p -p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (CST, D13.14.4E, 4370S, 1:200), p -p38 MAPK (Thr180/Tyr182) (CST, D3F9, 4511S, 1:200), p -Akt (Ser473) (CST, D9E, 4060S, 1:200), CD3ε (CST, D7A6E, 85061S, 1:200), CD4 (Abcam, ab133616, 1:400), CD8α (CST, D8A8Y, 85336S, 1:400), CD14 (Abcam, ab182032, 1:400), CD68 (CST, D4B9C, 76437S, 1:400), CD11b (CST, D6X1N, 49420S, 1:200), CD11c (Abcam, ab52632, 1:200), CD20 (Abcam, ab64088, 1:200), Foxp3 (CST, D2W8E, 98377S, 1:100), CD56 (CST, 123C3, 3576S, 1:100), TIM-3 (CST, D5D5R, 45208S, 1:50), TIGIT (Abcam, ab243903, 1:200), PD-1 (CST, EH33, 43248S, 1:100).

Techniques: Expressing, Immunohistochemical staining, Computed Tomography, Magnetic Resonance Imaging, Positron Emission Tomography-Computed Tomography, Positron Emission Tomography

Journal: Cureus

Article Title: Cardioprotective Effects of Sodium-Glucose Cotransporter Subtype Inhibition on Ischemic and Pharmacological Preconditioning

doi: 10.7759/cureus.59757

Figure Lengend Snippet: Lanes 1–3: Phl group. Lanes 4–6: IPC group. Red arrow: EGFR. Loading control: detection with anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies. IPC, ischemic preconditioning; Phl, phlorizin.

Article Snippet: Western blotting Phospho-AMPK and epidermal growth factor receptor (EGFR) levels were assessed using immunoblotting with specific isoform antibodies (cat# 2535 and 2646, Cell Signaling Technology, Danvers, MA).

Techniques: Control